8RY2
Crystal Structure of ANV419, a novel IL-2/anti-IL-2 antibody fusion protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-15 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 |
Unit cell lengths | 37.080, 90.260, 96.370 |
Unit cell angles | 87.02, 79.83, 88.23 |
Refinement procedure
Resolution | 47.370 - 2.050 |
R-factor | 0.192 |
Rwork | 0.190 |
R-free | 0.22400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.7.17) |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.370 | 47.370 | 2.100 |
High resolution limit [Å] | 2.050 | 9.170 | 2.050 |
Rmerge | 0.055 | 0.022 | 0.932 |
Rmeas | 0.068 | 0.027 | 1.186 |
Number of reflections | 74225 | 820 | 4847 |
<I/σ(I)> | 10.31 | 40.38 | 0.86 |
Completeness [%] | 96.4 | 95.6 | 86.4 |
Redundancy | 2.615 | 2.467 | 2.046 |
CC(1/2) | 0.999 | 0.998 | 0.656 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 80mM NaCl, 80mM Bicine, 15.54% PEG MME 550, 15% glycerol |