8RY2

Crystal Structure of ANV419, a novel IL-2/anti-IL-2 antibody fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-15
Detector	PSI PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	37.080, 90.260, 96.370
Unit cell angles	87.02, 79.83, 88.23

Refinement procedure

Resolution	47.370 - 2.050
R-factor	0.192
Rwork	0.190
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.17)
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.370	47.370	2.100
High resolution limit [Å]	2.050	9.170	2.050
Rmerge	0.055	0.022	0.932
Rmeas	0.068	0.027	1.186
Number of reflections	74225	820	4847
<I/σ(I)>	10.31	40.38	0.86
Completeness [%]	96.4	95.6	86.4
Redundancy	2.615	2.467	2.046
CC(1/2)	0.999	0.998	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	80mM NaCl, 80mM Bicine, 15.54% PEG MME 550, 15% glycerol