8RXG
Crystal structure of alpha-keto C-methyl transferase SgvM bound to SAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000040 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.205, 67.205, 183.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.090 - 1.813 |
R-factor | 0.1966 |
Rwork | 0.195 |
R-free | 0.23710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.950 |
Data reduction software | autoPROC |
Data scaling software | autoPROC (1.0.5) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.086 | 1.844 |
High resolution limit [Å] | 1.813 | 1.813 |
Rmerge | 0.203 | 8.708 |
Rpim | 0.059 | |
Number of reflections | 39049 | 1880 |
<I/σ(I)> | 12.6 | 0.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 26.3 | 27.5 |
CC(1/2) | 0.999 | 0.350 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 100 mM Tris/HCl pH 8.5, 250 mM Li2SO4, and 23% PEG 3350 |