8RWW
Crystal structure of native alpha-keto C-methyl transferase SgvM bound to ketoleucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.278, 67.278, 182.967 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.090 - 1.950 |
R-factor | 0.1837 |
Rwork | 0.182 |
R-free | 0.21230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.090 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.252 | |
Rmeas | 0.257 | |
Rpim | 0.050 | |
Number of reflections | 31639 | 2168 |
<I/σ(I)> | 13.4 | |
Completeness [%] | 99.9 | |
Redundancy | 25.8 | |
CC(1/2) | 0.999 | 0.463 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 20% PEG 3350, 250 mM Li2SO4, and 100 mM Tris/HCl pH 8.5 |