8RVR

Crystal structure of Trypanosoma congolense pyruvate kinase in complex with a single-domain antibody (TcoPYK-sdAb42) in the presence of sulfate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-06-05
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 2
Unit cell lengths	167.520, 168.420, 177.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.320 - 3.190
R-factor	0.2234
Rwork	0.221
R-free	0.27470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.361
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.320	3.310
High resolution limit [Å]	3.190	3.190
Rmeas	0.327
Number of reflections	83505	13176
<I/σ(I)>	9.39
Completeness [%]	99.8
Redundancy	13.8
CC(1/2)	0.995	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM Bis-Tris propane pH 7.5, 200 mM sodium sulfate, 20% PEG 3350