8RUN

Structure of Oceanobacillus iheyensis group II intron in the presence of Li+, Mg2+, and ARN25850

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-05
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.92
Spacegroup name	P 21 21 21
Unit cell lengths	88.888, 95.155, 223.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.030 - 3.730
R-factor	0.17433
Rwork	0.171
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	2.100
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.030	3.868
High resolution limit [Å]	3.730	3.735
Number of reflections	20343	1962
<I/σ(I)>	16.15
Completeness [%]	99.7
Redundancy	13.3
CC(1/2)	1.000	0.602

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		303.15	100 mM Mg-Acetate, 200 mM KCl, 50 mM LiCl, 50 mM Na-HEPES pH 7.0, 4% PEG 8000