8RUJ
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+, 5'-exon, and ARN25850 after 1h soaking
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-12-11 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.92 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.570, 95.098, 227.801 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.520 - 3.120 |
R-factor | 0.19805 |
Rwork | 0.196 |
R-free | 0.23617 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 2.472 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.520 | 3.236 |
High resolution limit [Å] | 3.120 | 3.120 |
Number of reflections | 35276 | 3419 |
<I/σ(I)> | 11.9 | |
Completeness [%] | 99.0 | |
Redundancy | 13.5 | |
CC(1/2) | 0.998 | 0.316 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM magnesium acetate, 200 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 3% PEG8000 |