8RUH
Structure of Oceanobacillus iheyensis group II intron in the presence of K+, Mg2+ and 5'-exon
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-12-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.96 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.204, 96.484, 231.693 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 89.230 - 4.550 |
R-factor | 0.18835 |
Rwork | 0.184 |
R-free | 0.25782 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 1.333 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 89.230 | 4.713 |
High resolution limit [Å] | 4.550 | 4.550 |
Rmerge | 0.054 | 1.301 |
Number of reflections | 12132 | 1156 |
<I/σ(I)> | 11.08 | |
Completeness [%] | 99.7 | |
Redundancy | 6.3 | |
CC(1/2) | 0.998 | 0.573 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM magnesium acetate, 100 mM potassium chloride, 10 mM lithium chloride, 50 mM HEPES sodium, pH 7.0, 5% PEG8000 |