8RSX

TRYPTOPHAN SYNTHASE measured via serial crystallography from a silicon HARE-chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2023-06-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	183.600, 61.500, 67.600
Unit cell angles	90.00, 94.55, 90.00

Refinement procedure

Resolution	91.510 - 2.000
R-factor	0.1813
Rwork	0.179
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.906
Data reduction software	CrystFEL (0.10.2)
Data scaling software	CrystFEL (0.10.2)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.510	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	51012	5064
<I/σ(I)>	3.33	1.01
Completeness [%]	100.0	100
Redundancy	49.8
CC(1/2)	0.943	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	293	PEG 300, Tris-HCl, Cesium chloirde