8RST
Rap from bacteriophage Phi3T in presence of pheromone SRGHTS
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 60.843, 195.549, 92.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.890 - 2.560 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.25530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.382 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 2.655 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 18149 | 1784 |
| <I/σ(I)> | 20.73 | |
| Completeness [%] | 99.9 | |
| Redundancy | 8 | |
| CC(1/2) | 1.000 | 0.649 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1M Tris pH8.5, 15% glycerol ethoxylate, 4% tetrahydrofuran |
