8RSR

Crystal structure of marine actinobacteria clade rhodopsin (MAR) - human GTPase Arf1 (L8K,Q71L) chimera; N state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-21
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 1 2 1
Unit cell lengths	133.186, 39.787, 95.756
Unit cell angles	90.00, 124.54, 90.00

Refinement procedure

Resolution	39.440 - 2.300
R-factor	0.2547
Rwork	0.252
R-free	0.28220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.465
Data reduction software	XDS (20220820)
Data scaling software	STARANISO (2.3.91)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.440	39.440	2.190
High resolution limit [Å]	2.100	6.170	2.100
Rpim	0.068	0.024	1.279
Number of reflections	19820	990	992
<I/σ(I)>	6.4	22.4	0.6
Completeness [%]	93.0	95.2	71.5
Redundancy	3	2.9	2.9
CC(1/2)	0.997	0.997	0.172

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7	293	2.0 M Ammonium Phosphate