8RSK
Crystal structure of Methanobrevibacter oralis macrodomain in complex with Asn-ADPr
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.146, 110.443, 104.610 |
| Unit cell angles | 90.00, 119.56, 90.00 |
Refinement procedure
| Resolution | 69.955 - 2.359 |
| Rwork | 0.213 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.211 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.955 | 2.420 |
| High resolution limit [Å] | 2.359 | 2.360 |
| Number of reflections | 42263 | 3109 |
| <I/σ(I)> | 7.3 | 0.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.993 | 0.494 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 5 mM zinc acetate, 9% (w/v) PEG8000, 100 mM sodium cocadylate pH 6.5 |
