8RQW
Erk2 MAP kinase (T188E) AMP-PNP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.913, 70.601, 60.409 |
| Unit cell angles | 90.00, 109.61, 90.00 |
Refinement procedure
| Resolution | 44.300 - 1.800 |
| R-factor | 0.19777 |
| Rwork | 0.196 |
| R-free | 0.23058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.643 |
| Data reduction software | MOSFLM |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 35528 | 2110 |
| <I/σ(I)> | 7.8 | 0.9 |
| Completeness [%] | 89.9 | |
| Redundancy | 3 | |
| CC(1/2) | 0.979 | 0.587 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium hepes pH=7, 4% v/v PEG 400 and 2M ammonium sulfate. |
