8RQR

In meso structure of apolipoprotein N-acyltransferase, Lnt, from Escherichia coli in 7.10 monoacylglycerol

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS EIGER2 X CdTe 9M
Wavelength(s)	0.9686
Spacegroup name	P 21 2 21
Unit cell lengths	48.679, 76.461, 157.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.500 - 2.190
R-factor	0.2009
Rwork	0.199
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.798
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.500	2.270
High resolution limit [Å]	2.190	2.190
Number of reflections	30881	2997
<I/σ(I)>	7.7
Completeness [%]	99.1
Redundancy	6.4
CC(1/2)	0.990	0.310

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	100 mM MES pH 6.0, 8 %(v/v) MPD, and 50-400 mM sodium or potassium thiocyanate