8RQ9
Crystal structure of PROTAC CFT-1297 in complex with CRBN-midi and BRD4(BD2)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-24 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.882, 52.638, 130.348 |
| Unit cell angles | 96.44, 91.49, 99.22 |
Refinement procedure
| Resolution | 43.270 - 2.910 |
| R-factor | 0.2524 |
| Rwork | 0.249 |
| R-free | 0.29260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.895 |
| Data reduction software | xia2 |
| Data scaling software | xia2.multiplex |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 2.960 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Number of reflections | 20285 | 275 |
| <I/σ(I)> | 3.3 | |
| Completeness [%] | 97.8 | 75.6 |
| Redundancy | 6.6 | 6 |
| CC(1/2) | 0.990 | 0.260 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG 3350, 0.2 M sodium citrate and 0.1 M Bis-Tris Propane pH 7.5 |
