8RPT
Crystal structure of human DNPH1 mutant- D80A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-08 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 56.481, 65.584, 69.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.600 - 1.950 |
| R-factor | 0.1798 |
| Rwork | 0.178 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.871 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.640 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.182 | 1.476 |
| Rmeas | 0.193 | |
| Rpim | 0.520 | |
| Number of reflections | 19407 | 1320 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.8 | 17.5 |
| CC(1/2) | 0.999 | 0.836 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 8 % PEG 4000, 100 mM Sodium acetate pH 4.6 |
