8RPS
Crystal structure of human DNPH1 mutant- D80N bound to 5hmdUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-09 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.879, 56.828, 88.543 |
| Unit cell angles | 90.00, 106.45, 90.00 |
Refinement procedure
| Resolution | 42.460 - 1.750 |
| R-factor | 0.1806 |
| Rwork | 0.179 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.971 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.102 | 0.940 |
| Rmeas | 1.148 | |
| Rpim | 0.102 | 0.650 |
| Number of reflections | 36132 | 1975 |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.9 |
| CC(1/2) | 0.990 | 0.707 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293.15 | [25 mM sodium malonate, 37.5 mM imidazole and 37.5 mM boric acid] pH 4.0 and 15 % PEG 1500. |
