8RON
Crystal structure of human FAD synthase, isoform 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-10 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.754, 187.740, 79.383 |
Unit cell angles | 90.00, 96.45, 90.00 |
Refinement procedure
Resolution | 58.720 - 2.600 |
R-factor | 0.2181 |
Rwork | 0.215 |
R-free | 0.26830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.825 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.725 | 2.792 |
High resolution limit [Å] | 2.488 | 2.488 |
Number of reflections | 43831 | 2192 |
<I/σ(I)> | 9.8 | 1.5 |
Completeness [%] | 93.2 | 71.3 |
Redundancy | 6.6 | 7 |
CC(1/2) | 0.996 | 0.485 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 10% PEG 8000 and 10% ethylene glycol |