8ROL
Human cohesin SMC3-HD(EQ)/RAD21-N complex - ATP-Mg-bound conformation - Form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999997 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 201.067, 201.067, 92.376 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.640 - 3.110 |
| R-factor | 0.1899 |
| Rwork | 0.187 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.997 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.300 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Rmerge | 0.256 | 4.814 |
| Number of reflections | 20148 | 3129 |
| <I/σ(I)> | 17 | 1.03 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 39.1 | 39.1 |
| CC(1/2) | 0.999 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium chloride; 0.1 M Bicine pH 9.0; 20% w/v PEG 550 MME |
