8ROF
Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - ADP-Mg-bound conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-04 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999997 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.453, 113.906, 133.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.570 - 1.650 |
| R-factor | 0.1972 |
| Rwork | 0.196 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.113 | 2.391 |
| Number of reflections | 61059 | 9690 |
| <I/σ(I)> | 15.52 | 1.05 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 13.3 | 13.2 |
| CC(1/2) | 0.999 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M magnesium chloride; 0.1 M MES pH 6.0; 20 % w/v PEG 6000 |
