8RNI
HLA-A*03:01 with KRAS-G12V-10mer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 155.339, 155.339, 85.335 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.840 - 2.490 |
| R-factor | 0.2293 |
| Rwork | 0.227 |
| R-free | 0.27630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.004 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.840 | 2.576 |
| High resolution limit [Å] | 2.480 | 2.487 |
| Number of reflections | 21774 | 2014 |
| <I/σ(I)> | 20.73 | 1.99 |
| Completeness [%] | 98.6 | 94.24 |
| Redundancy | 1.145632406 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M ammonium citrate tribasic pH7, 12% PEG 3350 |
