8RMJ
Drosophila Semaphorin 2b in complex with glycosaminoglycan mimic SOS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-03 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 181.489, 181.489, 168.379 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.670 - 2.790 |
R-factor | 0.2478 |
Rwork | 0.246 |
R-free | 0.27380 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.546 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 157.170 | 2.820 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmeas | 0.086 | |
Number of reflections | 23325 | 1961 |
<I/σ(I)> | 21.6 | |
Completeness [%] | 96.4 | 100 |
Redundancy | 19.6 | 16.1 |
CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0, 5% PEG 6000 |