8RKS
Structure of VPS29-VPS35 bound to the LFa motif R21 of Fam21.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979070 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.267, 139.095, 146.147 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 146.150 - 3.100 |
| R-factor | 0.2555 |
| Rwork | 0.251 |
| R-free | 0.28770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.523 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 146.150 | 3.315 |
| High resolution limit [Å] | 2.972 | 2.972 |
| Rmerge | 0.166 | 1.576 |
| Rmeas | 0.180 | 1.703 |
| Rpim | 0.067 | 0.639 |
| Total number of observations | 11011 | |
| Number of reflections | 31911 | 1584 |
| <I/σ(I)> | 7.4 | 1.3 |
| Completeness [%] | 65.4 | |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M NaCl, 20% PEG3350 0.1 M Tris pH 8.5 |
