8RKE

Crystal structure of the complete N-terminal region of human ZP2 (hZP2-N1N2N3)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91840
Spacegroup name	C 1 2 1
Unit cell lengths	147.680, 97.770, 104.330
Unit cell angles	90.00, 94.80, 90.00

Refinement procedure

Resolution	21.920 - 2.700
R-factor	0.2297
Rwork	0.229
R-free	0.25780
Structure solution method	SIRAS
RMSD bond length	0.004
RMSD bond angle	0.775
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.19.2_4158+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.920	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.082	2.152
Rmeas	0.089	2.345
Rpim	0.034	0.916
Number of reflections	40494	3976
<I/σ(I)>	15.7	0.9
Completeness [%]	99.2	98
Redundancy	6.9	6.4
CC(1/2)	1.000	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	10% (w/v) PEG 4000, 0.1 M MES/imidazole pH 6.5, 0.1 M carboxylic acids (MD2-100-76, Molecular Dimensions), 20% (v/v) glycerol