8RIH
Crystal structure of the Saccharomyces cerevisiae URH1p riboside hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.87313 |
| Spacegroup name | P 63 |
| Unit cell lengths | 140.284, 140.284, 81.343 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 121.490 - 2.740 |
| Rwork | 0.186 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.908 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 121.490 | 2.890 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.167 | 0.988 |
| Number of reflections | 13763 | 7714 |
| <I/σ(I)> | 5.9 | 1.8 |
| Completeness [%] | 94.1 | 81.8 |
| Redundancy | 12.9 | 11.2 |
| CC(1/2) | 0.981 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM TrisHCl, 800 mM LiCl, 32% PEG 4000 |
