8RHA
HSPB7ACD C131S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.858, 57.858, 167.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.740 - 2.180 |
| R-factor | 0.25475 |
| Rwork | 0.253 |
| R-free | 0.27961 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.399 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.780 | 2.220 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.138 | 1.418 |
| Number of reflections | 15713 | 764 |
| <I/σ(I)> | 14.5 | 2.2 |
| Completeness [%] | 100.0 | 99.1 |
| Redundancy | 25.4 | 25.1 |
| CC(1/2) | 1.000 | 0.957 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M magnesium formate dihydrate 20% w/v polyethylene glycol (PEG) 3350 |
