8RGE

Serial synchrotron in plate room temperature structure of Lysozyme.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE VMXi
Synchrotron site	Diamond
Beamline	VMXi
Temperature [K]	298
Detector technology	PIXEL
Collection date	2023-05-18
Detector	DECTRIS EIGER2 X 4M
Wavelength(s)	0.775
Spacegroup name	P 43 21 2
Unit cell lengths	78.573, 78.573, 37.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.560 - 1.880
R-factor	0.1947
Rwork	0.193
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.903
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.560	1.947
High resolution limit [Å]	1.880	1.880
Number of reflections	10076	973
<I/σ(I)>	19.1	1.2
Completeness [%]	99.3
Redundancy	110.9
CC(1/2)	0.997	0.398

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3.5	295	1M acetic acid pH 3, 5 % Peg 6000, 20 % NaCl