8RD3

Crystal structure of Saccharomyces cerevisiae Nmd4 protein bound to Upf1 helicase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	119.350, 131.160, 169.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.800 - 2.402
R-factor	0.2029
Rwork	0.202
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.940
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.620
High resolution limit [Å]	2.400	2.400
Rpim	0.053	0.596
Number of reflections	38847	1942
<I/σ(I)>	11.6	1.3
Completeness [%]	74.6
Redundancy	40.8
CC(1/2)	0.998	0.509

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	35% Tacsimate pH 7