8RC7
The structure of membrane-active antibiotic cyclodecapeptide gramicidin S in complex with urea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1978-01-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 25.800, 25.800, 21.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.340 - 0.980 |
| Rwork | 0.140 |
| R-free | 0.13180 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.196 |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.340 |
| High resolution limit [Å] | 0.980 |
| Number of reflections | 4028 |
| <I/σ(I)> | 210 |
| Completeness [%] | 81.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Urea, HCl, 95% Ethanol |
