8RBU
Crystal structure of HLA-A*11:01 in complex with SVLNDILARL, an 10-mer epitope from SARS-CoV-2 Spike (S975-984)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953732 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 86.992, 86.992, 107.183 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.670 - 2.700 |
| R-factor | 0.2061 |
| Rwork | 0.204 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.403 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.670 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.175 | 0.985 |
| Rmeas | 0.184 | 1.036 |
| Rpim | 0.055 | 0.317 |
| Number of reflections | 13337 | 1734 |
| <I/σ(I)> | 13 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 10.8 |
| CC(1/2) | 0.996 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% PEG3350, 0.2M NaCl and 0.1M Tris HCl pH8.5 |
