8RBK
Bos taurus PRMT9 in the presence of SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980115 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.433, 121.247, 67.355 |
| Unit cell angles | 90.00, 113.32, 90.00 |
Refinement procedure
| Resolution | 45.970 - 3.600 |
| R-factor | 0.2409 |
| Rwork | 0.239 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.529 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.19.2) |
| Refinement software | PHENIX (1.19rc4_4035) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.970 | 3.940 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.105 | 2.446 |
| Rmeas | 0.114 | 2.650 |
| Rpim | 0.043 | 1.012 |
| Total number of observations | 90508 | 21045 |
| Number of reflections | 12994 | 3096 |
| <I/σ(I)> | 8.4 | 0.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 7 | 6.8 |
| CC(1/2) | 0.997 | 0.516 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM Tris pH 7.5 0.8 M A.S. 2.5% PEG 6,000 |
