8RAX

The crystal structure of DNA-bound human MutSbeta (MSH2_G674D/MSH3) in the canonical mismatch bound conformation, nucleotide free

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-29
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.999841
Spacegroup name	P 1
Unit cell lengths	66.899, 90.998, 96.162
Unit cell angles	67.68, 86.73, 73.86

Refinement procedure

Resolution	75.930 - 3.162
R-factor	0.25901
Rwork	0.257
R-free	0.29247
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	1.260
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.930	3.217
High resolution limit [Å]	3.162	3.162
Number of reflections	31196	1540
<I/σ(I)>	9.7
Completeness [%]	90.7
Redundancy	2.1
CC(1/2)	0.997	0.786

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.1 M MES pH 6.5 -7.5, 0.2 M Ammonium Acetate, 20 - 25 % PEG 3350