8R9T

Crystal structure of the C-terminal domain of Chlamydomonas reinhardtii CFAP410

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97242
Spacegroup name	P 21 21 21
Unit cell lengths	43.435, 48.097, 53.156
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.790 - 1.400
R-factor	0.1649
Rwork	0.163
R-free	0.18860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.136	1.019
Rmeas	0.142	1.074
Rpim	0.040	0.331
Number of reflections	22256	19011
<I/σ(I)>	9.4	1.11
Completeness [%]	98.2	87.57
Redundancy	12.5	9.9
CC(1/2)	0.995	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.02 M CaCl2, 0.1 M sodium acetate (pH 5.0), 30% (v/v) 2-Methyl-2,4-pentanediol