8R9R
GDP-bound state of S. putrefaciens FlhF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 273 |
| Detector technology | PIXEL |
| Collection date | 2022-11-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.885600 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 73.920, 73.920, 159.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.970 - 2.420 |
| R-factor | 0.20844 |
| Rwork | 0.206 |
| R-free | 0.25034 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.970 | 2.507 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.114 | 2.228 |
| Rmeas | 0.116 | 2.258 |
| Rpim | 0.020 | 0.361 |
| Number of reflections | 100474 | 1001 |
| <I/σ(I)> | 21.75 | |
| Completeness [%] | 99.8 | |
| Redundancy | 35.8 | |
| CC(1/2) | 1.000 | 0.754 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 1 M lithium chloride, 0.1 M citric acid, 20% PEG 6000 |
