8R6B

DTX1 WWE domain in complex with ADP bound to WWE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999979
Spacegroup name	P 21 21 21
Unit cell lengths	33.856, 67.372, 70.291
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.150 - 2.500
R-factor	0.222
Rwork	0.221
R-free	0.24950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.715
Data reduction software	XDS (Feb 5, 2021)
Data scaling software	autoPROC (1.1.7)
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.290	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.109
Number of reflections	5530	507
<I/σ(I)>	10.8	4
Completeness [%]	92.9	77.4
Redundancy	5.9	3.9
CC(1/2)	0.991	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	0.1 M Tris pH 8.0, 25 % v/v PEG MME 350