8R5D

Crystal structure of human TRIM7 PRYSPRY domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000030
Spacegroup name	P 21 21 21
Unit cell lengths	61.258, 70.905, 85.503
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.790 - 1.800
R-factor	0.20589
Rwork	0.204
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.496
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.350	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.113	0.898
Rmeas	0.122	0.973
Rpim	0.047	0.371
Total number of observations	237490	13884
Number of reflections	35213	2068
<I/σ(I)>	7.2	2
Completeness [%]	99.9
Redundancy	6.7	6.7
CC(1/2)	0.996	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M Sodium malonate dibasic monohydrate pH 7.0, 20 % PEG3350