8R5B
Crystal structure of human TRIM7 PRYSPRY domain bound to (3-phenoxybenzoyl)-L-glutamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 41.189, 87.436, 122.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.760 - 1.600 |
| R-factor | 0.15028 |
| Rwork | 0.148 |
| R-free | 0.18684 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.634 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.760 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.075 | 0.255 |
| Rmeas | 0.082 | 0.276 |
| Rpim | 0.031 | 0.106 |
| Total number of observations | 19038 | |
| Number of reflections | 59265 | 2879 |
| <I/σ(I)> | 13.8 | 6.1 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 | 6.6 |
| CC(1/2) | 0.997 | 0.964 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 2M NaCl 100 mM acetate pH 4.5 |
