8R3P
Transketolase from Enterococcus faecium in complex with thiamin pyrophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-05 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 249.660, 68.220, 165.460 |
Unit cell angles | 90.00, 110.71, 90.00 |
Refinement procedure
Resolution | 34.940 - 2.900 |
R-factor | 0.2094 |
Rwork | 0.208 |
R-free | 0.24330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.804 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.940 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 57466 | 5558 |
<I/σ(I)> | 6.41 | |
Completeness [%] | 98.4 | |
Redundancy | 2.76 | |
CC(1/2) | 0.986 | 0.607 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M MgCl2 0.1 M Tris pH 8.5, 16 % (w/v) PEG 4000 |