8R2P
YZwIdeal x16 a scaffold for cryo-EM of small proteins of interest crystallizing in space group 19 (P 21 21 21)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.976246 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.260, 196.760, 208.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.910 - 2.220 |
| R-factor | 0.18846 |
| Rwork | 0.186 |
| R-free | 0.23039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.910 | 2.425 |
| High resolution limit [Å] | 2.217 | 2.217 |
| Rmerge | 0.167 | 1.041 |
| Rmeas | 0.182 | 1.156 |
| Rpim | 0.070 | 0.490 |
| Number of reflections | 61481 | 3073 |
| <I/σ(I)> | 8.1 | 1.68 |
| Completeness [%] | 41.1 | 8.8 |
| Redundancy | 6.6 | 5.12 |
| CC(1/2) | 0.997 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 50% w/v PEG200, 0.2M MgCl2 and 0.1M Sodium Cacodylate buffer pH 6.5 |
