8R17
Crystal structure of Neurospora crassa NADase with modified C-terminus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-12 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 1.033 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.756, 83.756, 103.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.940 - 2.300 |
R-factor | 0.2349 |
Rwork | 0.234 |
R-free | 0.25780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.758 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.21rc1_5109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.980 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.048 | 1.828 |
Rpim | 0.018 | 0.666 |
Number of reflections | 16949 | 1626 |
<I/σ(I)> | 20.1 | 1.3 |
Completeness [%] | 100.0 | |
Redundancy | 8.6 | |
CC(1/2) | 0.999 | 0.442 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Bis-Tris pH 5.5-6.5, NaCl |