8QZO
Crystal structure of heterodimeric complex of CdpB1 and CdpB2 from A. fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.086, 102.633, 112.323 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.490 - 2.290 |
R-factor | 0.2337 |
Rwork | 0.231 |
R-free | 0.27890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.028 |
Data reduction software | AutoProcess |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.760 | 2.430 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmeas | 0.088 | 1.618 |
Number of reflections | 30565 | 1527 |
<I/σ(I)> | 11.3 | |
Completeness [%] | 89.0 | |
Redundancy | 6 | |
CC(1/2) | 0.999 | 0.458 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | MPD 5% v/v, magnesium chloride hexahydrate 0.04M, sodium cacodylate trihydrate 0.05M pH 6 |