8QZN

DARPin-Armadillo fusion protein with a selected Tyrosin pocket binding to the fused target peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99986
Spacegroup name	P 21 21 21
Unit cell lengths	50.850, 93.610, 76.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 1.400
R-factor	0.1694
Rwork	0.169
R-free	0.18010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.643
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	1.440
High resolution limit [Å]	1.400	1.400
Number of reflections	72887	5305
<I/σ(I)>	15
Completeness [%]	99.9	99.9
Redundancy	13.1
CC(1/2)	0.998	0.499

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	2 M (NH4)2SO4, 0.1 M Bis-Tris pH 5.5