8QZ4

Crystal structure of human two pore domain potassium ion channel TREK-2 (K2P10.1) in complex with an activatory nanobody (Nb76)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9687
Spacegroup name	P 21 21 21
Unit cell lengths	68.280, 75.576, 259.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.110 - 3.200
R-factor	0.2718
Rwork	0.270
R-free	0.29840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.650
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.830	3.336
High resolution limit [Å]	3.203	3.203
Rmeas	0.134	1.065
Rpim	0.066	0.522
Number of reflections	17904	892
<I/σ(I)>	8.4	1.7
Completeness [%]	77.9	34.4
Redundancy	4	4
CC(1/2)	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M HEPES pH 7.0, 0.3 M BaCl2, 39% polyethylene glycol 400