8QYP

Beta-cardiac myosin motor domain in the pre-powerstroke state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98007
Spacegroup name	P 43 2 2
Unit cell lengths	94.397, 94.397, 219.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.400 - 2.759
R-factor	0.1926
Rwork	0.190
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.020
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (18-SEP-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.400	3.033
High resolution limit [Å]	2.759	2.759
Rmerge	0.273	2.413
Number of reflections	20166	1009
<I/σ(I)>	10.8	1.5
Completeness [%]	94.4	64.2
Redundancy	25.9
CC(1/2)	0.999	0.710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.5M Lithium Sulfate 0.1M Tris-HCl 25% PEG 3350 2mM Mg.ADP.Vanadate