8QYB
J22.9-ISY, fully humanized and CDR optimized Fab Fragment based on chimeric J22.9-xi IgG against BCMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.185, 54.617, 81.084 |
| Unit cell angles | 90.00, 121.09, 90.00 |
Refinement procedure
| Resolution | 45.250 - 3.090 |
| R-factor | 0.2495 |
| Rwork | 0.247 |
| R-free | 0.30560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.486 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 3.205 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmeas | 0.250 | 0.996 |
| Number of reflections | 9514 | 1380 |
| <I/σ(I)> | 5.15 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.975 | 0.728 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% PEG 3350 7.5 mM CuCl2 BisTris |
