8QVD
Deinococcus aerius TR0125 C-glucosyl deglycosidase (CGD), wild type crystal cryoprotected with glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-01-27 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.953731 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.030, 93.780, 93.460 |
Unit cell angles | 90.00, 94.94, 90.00 |
Refinement procedure
Resolution | 49.510 - 3.300 |
R-factor | 0.2443 |
Rwork | 0.239 |
R-free | 0.29650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.434 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.510 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.193 | |
Number of reflections | 18261 | 1557 |
<I/σ(I)> | 7.4 | 1.2 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 6.9 | 7.1 |
CC(1/2) | 0.997 | 0.637 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 12% (w/v) polyethylene glycol 3,350, 5 mM CoCl2, 5 mM CdCl2, 5 mM MgCl2, and 5 mM NiCl2 |