8QV7
Crystal structure of human TDO with alpha-methyl-L-tryptophan
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-27 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.285, 90.756, 132.774 |
| Unit cell angles | 90.00, 120.75, 90.00 |
Refinement procedure
| Resolution | 34.460 - 2.660 |
| R-factor | 0.2283 |
| Rwork | 0.227 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.100 | 2.860 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.191 | 0.559 |
| Rpim | 0.122 | 0.354 |
| Number of reflections | 31931 | 1597 |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 90.7 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.971 | 0.730 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20 mg/lml protein 50 mM HEPES pH 8.0 200 mM NaCl 2mM TCEP 10% glycerol |
