8QU7

Crystal structure of the Plasmodium falciparum Apical membrane antigen (AMA1) in complex with single domain i-body WD34

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	I 1 2 1
Unit cell lengths	71.651, 38.462, 227.355
Unit cell angles	90.00, 90.18, 90.00

Refinement procedure

Resolution	38.450 - 2.600
R-factor	0.2143
Rwork	0.213
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.603
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.450	2.693
High resolution limit [Å]	2.600	2.600
Number of reflections	19822	2335
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	4.8
CC(1/2)	0.994	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1MSodium HEPES pH7.5 70% v/v MPD