8QTC

Crystal structure of Arabidopsis thaliana 14-3-3 omega in complex with a phosphopeptide from the transcription factor BZR1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-06
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999999406361
Spacegroup name	P 65 2 2
Unit cell lengths	131.503, 131.503, 256.357
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	68.350 - 3.500
R-factor	0.2184
Rwork	0.217
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.350	3.710
High resolution limit [Å]	3.500	3.500
Rmeas	0.339	5.050
Number of reflections	17253	2692
<I/σ(I)>	14.32	0.91
Completeness [%]	99.9
Redundancy	38.3	39.6
CC(1/2)	1.000	0.370

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	2 M (NH4)2SO4 , 10 mM CoCl2 6 H2O, 0.1 M Mes (pH 6.5)