8QLL
Crystal structure of rat glutathione transferase Omega 1 bound to glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM07 |
Synchrotron site | ESRF |
Beamline | BM07 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97951 |
Spacegroup name | I 4 |
Unit cell lengths | 133.164, 133.164, 88.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.080 - 2.400 |
R-factor | 0.2544 |
Rwork | 0.252 |
R-free | 0.29580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.978 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.410 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.103 | 0.656 |
Rmeas | 0.111 | 0.711 |
Rpim | 0.043 | 0.272 |
Number of reflections | 30224 | 3048 |
<I/σ(I)> | 8.1 | 1 |
Completeness [%] | 99.5 | |
Redundancy | 6.9 | |
CC(1/2) | 0.996 | 0.896 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 2 M ammonium sulfate, 100 mM Na acetate pH 4.6, 10 mM glutathione |