8QK8
Structure of Legionella pneumophila Lcl C-terminal domain bound to sulphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 74.910, 74.910, 94.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.874 - 1.900 |
| Rwork | 0.160 |
| R-free | 0.18740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.910 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 0.541 |
| Number of reflections | 24856 | 1235 |
| <I/σ(I)> | 28.7 | 5.8 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 19.3 | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% (v/v) glycerol, 20% (w/v) polyethylene glycol (PEG) 4000, 30 mM NaNO3, 30 mM Na2HPO4, 30 mM (NH4)2SO4, 100 mM bicine, 100 mM tris-HCl |
